 | Related Articles |
Purification of Tetrahymena cytoskeletal proteins.
Methods Cell Biol. 2012;109:379-91
Authors: Honts JE
Abstract
Like all eukaryotic cells, Tetrahymena thermophila contains a rich array of cytoskeletal proteins, some familiar and some novel. A detailed analysis of the structure, function, and interactions of these proteins requires procedures for purifying the individual protein components. Procedures for the purification of actin and tubulin from Tetrahymena are reviewed, followed by a description of a procedure that yields proteins from the epiplasmic layer and associated structures, including the tetrins. Finally, the challenges and opportunities for future advances are assessed.
PMID: 22444153 [PubMed - indexed for MEDLINE]
No comments:
Post a Comment