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Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements.
J Biotechnol. 2012 Jan;157(1):167-72
Authors: Phan TT, Nguyen HD, Schumann W
Abstract
Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the -35, -15, -10 and the +1 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein.
PMID: 22100269 [PubMed - indexed for MEDLINE]
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