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Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd(2+).
Bioorg Med Chem Lett. 2012 Jun 15;22(12):4014-7
Authors: Li Y, Li L, Pu X, Ma G, Wang E, Kong J, Liu Z, Liu Y
Abstract
A novel ratiometric fluorescent peptidyl chemosensor (Dansyl-Cys-Pro-Gly-Cys-Trp-NH(2), D-P5) for metal ions detection has been synthesized via Fmoc solid-phase peptide synthesis. The chemosensor exhibited a high selectivity for Cd(2+) over other metal ions including competitive transition and Group I and II metal ions in neutral pH. The fluorescence emission intensity of D-P5 was significantly enhanced in the presence of Cd(2+) by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The binding stoichiometry, detection limit, binding affinity, reversibility and pH sensitivity of the sensor for Cd(2+) were investigated.
PMID: 22579421 [PubMed - in process]
Source: http://www.ncbi.nlm.nih.gov/PubMed/22579421?dopt=Abstract
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